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Complete knockout of endogenous Mdr1 (Abcb1) in MDCK wild type and MDCK-hMDR1 cells by CRISPR-Cas9
- In: Poster Presentation
- At: Stockholm (Sweden) (2017)
- Type: Poster
- Poster code: P-A-073-Tuesday
- By: KARLGREN, Maria (Uppsala University, Department of Pharmacy, Uppsala, Sweden)
- Co-author(s): Maria Karlgren: Department of Pharmacy, Uppsala University, Uppsala, Sweden;Uppsala University Drug Optimization and Pharmaceutical Profiling Platform (UDOPP), Science for Life Laboratory, Uppsala, Sweden
Ivailo Simoff: Department of Pharmacy, Uppsala University, Uppsala, Sweden
Maria Backlund: Department of Pharmacy, Uppsala University, Uppsala, Sweden;Uppsala University Drug Optimization and Pharmaceutical Profiling Platform (UDOPP), Science for Life Laboratory, Uppsala, Sweden
Anne-Christine Lindström: Department of Immunology, Genetics and Pathology, Science for Life Laboratory, Uppsala University, Uppsala, Sweden
Christine Wegler: Department of Pharmacy, Uppsala University, Uppsala, Sweden;Cardiovascular Metabolic Diseases DMPK, AstraZeneca R&D, Mölndal, Sweden
Niklas Handin: Department of Pharmacy, Uppsala University, Uppsala, Sweden
Patrik Lundquist: Department of Pharmacy, Uppsala University, Uppsala, Sweden
Markus Keiser: Department of Clinical Pharmacology, Center of Drug Absorption and Transport (C_DAT), University Medicine of Greifswald, Greifswald, Germany
Stefan Oswald: Department of Clinical Pharmacology, Center of Drug Absorption and Transport (C_DAT), University Medicine of Greifswald, Greifswald, Germany
Per Artursson: Department of Pharmacy, Uppsala University, Uppsala, Sweden;Uppsala University Drug Optimization and Pharmaceutical Profiling Platform (UDOPP), Science for Life Laboratory, Uppsala, Sweden - Abstract:
Backgrounds
Madin-Darby canine kidney (MDCK) II cells transfected with transport proteins are commonly used for studying drug transport. However, endogenous transporters e.g. canine Mdr1 (Abcb1) complicates the interpretation of transport studies.Aims
The aim of this study was to establish MDCK cell lines lacking endogenous canine Mdr1 (cMdr1).. The access to the whole abstract and if available the presentation file is available to FIP members and to congress participants of that specific congress.
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Last update 28 September 2023